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Determination of calcium from irrigation water sample.

Aim -
Determination of Calcium from irrigation water sample.
Principle -
When disodium salt of is added to water containing both calcium + magnesium is a determine separately with calcon indicator with 10% solution. Sodium EDTA forms a stronger Complex with calcium the the solution changes from Pink to blue colour. Record the volume of standard edit for calculating calcium in water. 
Reagents -
1) EDTA SOLUTION (0.01N) -
Weigh 2 gram versenate (disodium hydrogen EDTA) dissolve it in distilled water make up volume 1 litre. 

2) Sodium hydroxide (10%) -
10 gram is NaoH dissolved in distilled water and make the volume hundred ml.

3) Calcon indicator -
 Dissolve 20 ml gram of calcon in 50 ml of methanol (or ethanol). 

4) Potassium ferrocyanide (K4fe(cn)6) -
Dissolve 4 gm potassium ferrocyanide in hundred ml distilled water. 

5)Hydroxylamine hydrochoride (NH4oH. Hcl) -
Dissolved 5 gram hydroxylamine hydrochloride in hundred ml distilled water. 

6)Triethanolamine -
TEA indicator reagent grade.

Procedure -
  •  Take 10ml of water sample in clean hundred ml conical flask and add 25 ml distilled water. 
  • Add 10 drops of each hydroxylamine hydrochloride potassium ferrocyanide and triethanolamine. 
  • Add approximately 5ml or enough 10% NaoH solution to raise PH to 12 and 5 drops of calcon indicator prepared freshly shake the content well. 
  • Titrate the content with standard EDTA solution till pink colour changes blue. 
  • Record the volume of standard EDTA used.
  • Run the blank simultaneously using 10 ml distilled water in place of sample. 
  • Use all Regent and titrate. 
Subtract blank reading from sample reading to get the true reading off at the sample.

Calculation
Sample reading  - blank reading  = true reading  of EDTA required. 

Formula 
ca²++ m.eq./lit. = 
N. of EDTA × volume of EDTA required ×1000
÷ volume of aliquote taken. 

Ca²+ m.eq./lit.  = 0.01 × volume of EDTA required × 1000
÷ volume of aliquote taken. 

Ca++ m.eq ./lit. =  -------

Results 
Calcium of given water sample is -------m. eq./lit. 

Now we can easily calculate mg²+ by subtracting value of ca²+ from (ca²+ + mg² +)





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